RESULTS The shearing forces, such as vortex, high speed centrifugation or stirring, had little influences on the activity of Newcastle disease virus. 结果涡旋、高速离心和搅拌等剪切力时新城疫病毒的活性影响不大;超声时病毒活性产生一定的影响;
The electromagnetic bearing used on high speed centrifugation driers is established, and an electric control system is used with a core of numeric controller, using PID control arithmetic. The control parameters used in guiding system debugging are optimized from system simulation. 研制应用在高速离心干燥机上的电磁轴承,开发以数字控制器为核心的电控系统,采用PID控制算法,通过仿真获得的参数指导实际调试,实现控制参数的最优化。
Baker's yeast were treated with ultrasonic crash, high speed centrifugation, ammonium sulfate precipitation, dialysis and freeze-drying to gain the raw dehydrogenases, which then were purified by dye affinity chromatography. 将面包酵母经超声破碎、高速离心、硫酸铵分级沉淀、透析、冰冻干燥等步骤得到脱氢酶粗品,再经染料亲和层析柱进一步纯化。
The strain was cultured under light treatment, temperature 38 ℃, shake cultivation 6 days, the purple membrane was gained through high speed freeze centrifugation, dialysis, sucrose density gradient centrifugation, superspeed centrifugation etc. 该菌株在光照条件下,38℃摇瓶培养6d,可用高速冷冻离心、透析袋、蔗糖密度梯度纯化、超速离心等得到紫膜。
Results: the method of high speed centrifugation could shorten preparing technological process, and much more contents obtained by it than that was obtained by the normal method of water ethanol method. 结果:高速离心法缩短工艺流程,含量也明显高于常规水醇方法。
Method: To prepare Zhike Dingchuan Koufuye with different method of high speed centrifugation and water ethanol method, then measure the respective content obtained by those two different methods. 方法:采用高速离心法与水醇法同时制备止咳定喘口服液,并测定其含量。
The experiments of long-time, high speed, high temperature running, which have been done in lab and factories, shows that the system has meet the need of application of high speed centrifugation driers. 在实验室和工厂进行的高温高速加载运转实验表明,研究的电磁轴承已经达到高速离心干燥机的应用要求。
Conclusion: the preparing method of high speed centrifugation could replace the normal preparing method of water ethanol method. 结论:高速离心法可取代常规水醇制备方法。
The protein in serum sample was precipitated by methanol, and the supernatant was directly injected into HPLC after high speed centrifugation. 血清标本经甲醇沉淀蛋白,高速离心后取上清液直接进样。
The cell membrane fractions were collected by high speed centrifugation. valbyensis菌体,收集后的菌体用液氮研磨破碎,再用高速离心收集细胞膜片段。
A kind of hepatocyte growth factor was purified from cow placenta through high speed centrifugation, salting out, affinity chromatography, ultrafiltration and anion exchange chromatography. 用高速离心、分段盐析、亲和层析、超滤和阴离子交换层析法,从牛胎盘中分离纯化出一种肝细胞生长因子。
In high speed centrifugation condition, the centrifugation temperature and NaCl ionic intension of reverse extraction solution were changed to study their effects on the separation degree and the interaction of protein and AOT, and the optimum centrifugation temperature and NaCl addition were selected. 研究了在高速离心作用下,反萃液的离心温度和盐离子强度对AOT和蛋白质的分离程度及相互作用的影响,并确定最佳离心温度和盐离子强度。